Astragalus Polysaccharide Improves Insulin Sensitivity via AMPK Activation in 3T3-L1 Adipocytes

Astragalus Polysaccharide Improves Insulin Sensitivity via AMPK Activation in 3T3-L1 Adipocytes

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Astragalus polysaccharide (APS) is an important bioactive component of Astragalus membranaceus which is used as an anti-diabetes herb in traditional Chinese medicine.The objective mac miller kids poster of this study was to investigate the effects and mechanisms of APS on insulin-sensitizing of adipocytes.Mouse 3T3-L1 preadipocytes were used as a model.The results showed that APS increased preadipocytes proliferation in a dose dependent manner, and 0.

1 μg/mL APS sufficiently increased Proliferating Cell Nuclear Antigen (PCNA) content (p < 0.01).Moreover, APS enhanced intracellular lipid accumulation and mRNA expression of proliferator-activated receptor γ (PPARγ, p < 0.01), CCAAT/enhancer binding protein α (C/EBPα, p < 0.

01) and fatty acid binding protein (aP2, p < 0.01).As expected, corresponding protein contents were elevated.Importantly, APS increased 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) uptake (p < 0.

01).Meanwhile, both mRNA and protein content of glucose transporter 4 (Glut4) were elevated by APS (p < 0.01).The APS treatment enhanced tyrosine phosphorylation of insulin receptor substrate gold ps5 controller skin 1 (IRS1, p < 0.

05) and phosphor-Akt content (p < 0.01).Besides, phosphorylated AMP-activated protein kinase (AMPK) content was increased in the APS treated cells (p < 0.01).

Taken together, APS improved insulin sensitivity by enhancing glucose uptake, possibly through AMPK activation.These results suggested that APS might be a therapeutic candidate for insulin resistance.